Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

The CRISPR/Cas9 system is a robust genome editing technology that works in human cells, animals and plants based on the RNA-programmed DNA cleaving activity of the Cas9 enzyme. Building on previous work (Jinek et al., 2013), we show here that new genetic information can be introduced site-specifical...

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Detalles Bibliográficos
Autores principales: Lin, Steven, Staahl, Brett T, Alla, Ravi K, Doudna, Jennifer A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2014
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383097/
https://www.ncbi.nlm.nih.gov/pubmed/25497837
http://dx.doi.org/10.7554/eLife.04766

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383097/
https://www.ncbi.nlm.nih.gov/pubmed/25497837
http://dx.doi.org/10.7554/eLife.04766

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