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Dramatic Improvement of Proteomic Analysis of Zebrafish Liver Tumor by Effective Protein Extraction with Sodium Deoxycholate and Heat Denaturation

Majority of the proteomic studies on tissue samples involve the use of gel-based approach for profiling and digestion. The laborious gel-based approach is slowly being replaced by the advancing in-solution digestion approach. However, there are still several difficulties such as difficult-to-solubil...

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Detalles Bibliográficos
Autores principales: Wang, Jigang, Lee, Yew Mun, Li, Caixia, Li, Ping, Li, Zhen, Lim, Teck Kwang, Gong, Zhiyuan, Lin, Qingsong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383156/
https://www.ncbi.nlm.nih.gov/pubmed/25873971
http://dx.doi.org/10.1155/2015/763969