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Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation

A semiautomated extraction protocol of HCV-RNA using Favorgen RNA extraction kit has been developed. The kit provided protocol was modified by replacing manual spin steps with vacuum filtration. The assay performance was evaluated by real-time qPCR based on Taqman technology. Assay linearity was con...

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Detalles Bibliográficos
Autores principales: Amin, Imran, Jabbar, Tania, Niazi, Fawad, Akhtar, Muhammad Saeed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383304/
https://www.ncbi.nlm.nih.gov/pubmed/25873968
http://dx.doi.org/10.1155/2015/367801