Ratiometric Analysis of Fura Red by Flow Cytometry: A Technique for Monitoring Intracellular Calcium Flux in Primary Cell Subsets

Calcium flux is a rapid and sensitive measure of cell activation whose utility could be enhanced with better techniques for data extraction. We describe a technique to monitor calcium flux by flow cytometry, measuring Fura Red calcium dye by ratiometric analysis. This technique has several advantage...

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Detalles Bibliográficos
Autores principales: Wendt, Emily R., Ferry, Helen, Greaves, David R., Keshav, Satish
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383592/
https://www.ncbi.nlm.nih.gov/pubmed/25835294
http://dx.doi.org/10.1371/journal.pone.0119532

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383592/
https://www.ncbi.nlm.nih.gov/pubmed/25835294
http://dx.doi.org/10.1371/journal.pone.0119532

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