Methods to calibrate and scale axial distances in confocal microscopy as a function of refractive index

Accurate distance measurement in 3D confocal microscopy is important for quantitative analysis, volume visualization and image restoration. However, axial distances can be distorted by both the point spread function (PSF) and by a refractive-index mismatch between the sample and immersion liquid, wh...

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Detalles Bibliográficos
Autores principales: BESSELING, TH, JOSE, J, BLAADEREN, A VAN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2015
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383648/
https://www.ncbi.nlm.nih.gov/pubmed/25444358
http://dx.doi.org/10.1111/jmi.12194

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383648/
https://www.ncbi.nlm.nih.gov/pubmed/25444358
http://dx.doi.org/10.1111/jmi.12194

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