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Fine-tuning Interaction between Aminoacyl-tRNA Synthetase and tRNA for Efficient Synthesis of Proteins Containing Unnatural Amino Acids

[Image: see text] By using a directed evolution approach, we have identified aminoacyl-tRNA synthetase variants with significantly enhanced activity for the incorporation of unnatural amino acids into proteins in response to the amber nonsense codon in bacteria. We demonstrated that the optimization...

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Detalles Bibliográficos
Autores principales: Wang, Nanxi, Ju, Tong, Niu, Wei, Guo, Jiantao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384836/
https://www.ncbi.nlm.nih.gov/pubmed/24847685
http://dx.doi.org/10.1021/sb500195w