Frequency of Class 1 Integron in Escherichia Coli Strains Isolated from Patients with Urinary Tract Infections in North of Iran

BACKGROUND: Due to the importance of antibiotic resistance in E.coli and the possible role of integrons in creating of resistance, this study was performed to survey of class 1 integron in E. coli strains and their resistance to three routinely used antibiotics. METHODS: In this cross-sectional stud...

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Autores principales: Ahangarkani, Fatemeh, Rajabnia, Ramazan, Shahandashti, Elaheh Ferdosi, Bagheri, Meghdad, Ramez, Maryam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AVICENA, d.o.o., Sarajevo 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384837/
https://www.ncbi.nlm.nih.gov/pubmed/25870523
http://dx.doi.org/10.5455/msm.2014.27.10-12
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author Ahangarkani, Fatemeh
Rajabnia, Ramazan
Shahandashti, Elaheh Ferdosi
Bagheri, Meghdad
Ramez, Maryam
author_facet Ahangarkani, Fatemeh
Rajabnia, Ramazan
Shahandashti, Elaheh Ferdosi
Bagheri, Meghdad
Ramez, Maryam
author_sort Ahangarkani, Fatemeh
collection PubMed
description BACKGROUND: Due to the importance of antibiotic resistance in E.coli and the possible role of integrons in creating of resistance, this study was performed to survey of class 1 integron in E. coli strains and their resistance to three routinely used antibiotics. METHODS: In this cross-sectional study, 100 strains of E. coli were isolated from patients with Urinary tract infection. After diagnosis of bacteria, genomes were extracted. Then, presence of integron class 1 was evaluated by using PCR. Antibiotic susceptibility testing method, the micro dilution broth was performed according to the standard CLSI2010. Data were analyzed using SPSS16 software. RESULT: Out of the total number of 100 E. coli cases, 22 cases (22%) had class 1 integron. Resistance against cotrimoxazol, cefixime and ciprofloxacin antibiotics were 67%, 34% and 34% respectively. In 22 E. coli cases positive for integron class1 gene, resistance against three antibiotics were 100%, 95.45% and 90.90% respectively, which is statistically significant (p < 0.05). CONCLUSION: Resistance level against antibiotics in samples containing class 1 integron were significantly higher than those lacking this gene, which may be confirm the present of class 1 integron in creation of clinical strains with resistance to this antibiotics. Using suitable antibiotics may be preventing transmission of resistance genes through integrons.
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spelling pubmed-43848372015-04-13 Frequency of Class 1 Integron in Escherichia Coli Strains Isolated from Patients with Urinary Tract Infections in North of Iran Ahangarkani, Fatemeh Rajabnia, Ramazan Shahandashti, Elaheh Ferdosi Bagheri, Meghdad Ramez, Maryam Mater Sociomed Original Paper BACKGROUND: Due to the importance of antibiotic resistance in E.coli and the possible role of integrons in creating of resistance, this study was performed to survey of class 1 integron in E. coli strains and their resistance to three routinely used antibiotics. METHODS: In this cross-sectional study, 100 strains of E. coli were isolated from patients with Urinary tract infection. After diagnosis of bacteria, genomes were extracted. Then, presence of integron class 1 was evaluated by using PCR. Antibiotic susceptibility testing method, the micro dilution broth was performed according to the standard CLSI2010. Data were analyzed using SPSS16 software. RESULT: Out of the total number of 100 E. coli cases, 22 cases (22%) had class 1 integron. Resistance against cotrimoxazol, cefixime and ciprofloxacin antibiotics were 67%, 34% and 34% respectively. In 22 E. coli cases positive for integron class1 gene, resistance against three antibiotics were 100%, 95.45% and 90.90% respectively, which is statistically significant (p < 0.05). CONCLUSION: Resistance level against antibiotics in samples containing class 1 integron were significantly higher than those lacking this gene, which may be confirm the present of class 1 integron in creation of clinical strains with resistance to this antibiotics. Using suitable antibiotics may be preventing transmission of resistance genes through integrons. AVICENA, d.o.o., Sarajevo 2015-02 2015-02-21 /pmc/articles/PMC4384837/ /pubmed/25870523 http://dx.doi.org/10.5455/msm.2014.27.10-12 Text en Copyright: © Fatemeh Ahangarkani, Ramazan Rajabnia, Elaheh Ferdosi Shahandashti, Meghdad Bagheri, Maryam Ramez http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Paper
Ahangarkani, Fatemeh
Rajabnia, Ramazan
Shahandashti, Elaheh Ferdosi
Bagheri, Meghdad
Ramez, Maryam
Frequency of Class 1 Integron in Escherichia Coli Strains Isolated from Patients with Urinary Tract Infections in North of Iran
title Frequency of Class 1 Integron in Escherichia Coli Strains Isolated from Patients with Urinary Tract Infections in North of Iran
title_full Frequency of Class 1 Integron in Escherichia Coli Strains Isolated from Patients with Urinary Tract Infections in North of Iran
title_fullStr Frequency of Class 1 Integron in Escherichia Coli Strains Isolated from Patients with Urinary Tract Infections in North of Iran
title_full_unstemmed Frequency of Class 1 Integron in Escherichia Coli Strains Isolated from Patients with Urinary Tract Infections in North of Iran
title_short Frequency of Class 1 Integron in Escherichia Coli Strains Isolated from Patients with Urinary Tract Infections in North of Iran
title_sort frequency of class 1 integron in escherichia coli strains isolated from patients with urinary tract infections in north of iran
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384837/
https://www.ncbi.nlm.nih.gov/pubmed/25870523
http://dx.doi.org/10.5455/msm.2014.27.10-12
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