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Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival
The chloride channel-3 (ClC-3) protein is known to be a component of Cl(−) channels involved in cell volume regulation or acidification of intracellular vesicles. Here, we report that ClC-3 was highly expressed in the cytoplasm of metastatic carcinomatous cells and accelerated cell migration in vitr...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4385862/ https://www.ncbi.nlm.nih.gov/pubmed/25537517 |
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author | Xu, Bin Jin, Xiaobao Min, Ling Li, Qin Deng, Lulu Wu, Hui Lin, Guixian Chen, Lixin Zhang, Haifeng Li, Chunmei Wang, Liwei Zhu, Jiayong Wang, Weizhang Chu, Fujiang Shen, Juan Li, Hongzhi Mao, Jianwen |
author_facet | Xu, Bin Jin, Xiaobao Min, Ling Li, Qin Deng, Lulu Wu, Hui Lin, Guixian Chen, Lixin Zhang, Haifeng Li, Chunmei Wang, Liwei Zhu, Jiayong Wang, Weizhang Chu, Fujiang Shen, Juan Li, Hongzhi Mao, Jianwen |
author_sort | Xu, Bin |
collection | PubMed |
description | The chloride channel-3 (ClC-3) protein is known to be a component of Cl(−) channels involved in cell volume regulation or acidification of intracellular vesicles. Here, we report that ClC-3 was highly expressed in the cytoplasm of metastatic carcinomatous cells and accelerated cell migration in vitro and tumor metastasis in vivo. High-grade expression of cytoplasmic ClC-3 predicted poor survival in cancer patients. We found that independent of its volume-activated Cl(−) channel properties, ClC-3 was able to promote cell membrane ruffling, required for tumor metastasis. ClC-3 mediated membrane ruffling by regulating keratin 18 phosphorylation to control β1 Integrin recycling. Therefore, cytoplasmic ClC-3 plays an active and key role in tumor metastasis and may be a valuable prognostic biomarker and a therapeutic target to prevent tumor spread. |
format | Online Article Text |
id | pubmed-4385862 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-43858622015-04-14 Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival Xu, Bin Jin, Xiaobao Min, Ling Li, Qin Deng, Lulu Wu, Hui Lin, Guixian Chen, Lixin Zhang, Haifeng Li, Chunmei Wang, Liwei Zhu, Jiayong Wang, Weizhang Chu, Fujiang Shen, Juan Li, Hongzhi Mao, Jianwen Oncotarget Research Paper The chloride channel-3 (ClC-3) protein is known to be a component of Cl(−) channels involved in cell volume regulation or acidification of intracellular vesicles. Here, we report that ClC-3 was highly expressed in the cytoplasm of metastatic carcinomatous cells and accelerated cell migration in vitro and tumor metastasis in vivo. High-grade expression of cytoplasmic ClC-3 predicted poor survival in cancer patients. We found that independent of its volume-activated Cl(−) channel properties, ClC-3 was able to promote cell membrane ruffling, required for tumor metastasis. ClC-3 mediated membrane ruffling by regulating keratin 18 phosphorylation to control β1 Integrin recycling. Therefore, cytoplasmic ClC-3 plays an active and key role in tumor metastasis and may be a valuable prognostic biomarker and a therapeutic target to prevent tumor spread. Impact Journals LLC 2014-12-11 /pmc/articles/PMC4385862/ /pubmed/25537517 Text en Copyright: © 2015 Xu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Xu, Bin Jin, Xiaobao Min, Ling Li, Qin Deng, Lulu Wu, Hui Lin, Guixian Chen, Lixin Zhang, Haifeng Li, Chunmei Wang, Liwei Zhu, Jiayong Wang, Weizhang Chu, Fujiang Shen, Juan Li, Hongzhi Mao, Jianwen Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival |
title | Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival |
title_full | Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival |
title_fullStr | Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival |
title_full_unstemmed | Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival |
title_short | Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival |
title_sort | chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4385862/ https://www.ncbi.nlm.nih.gov/pubmed/25537517 |
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