Co-Solvents as Stabilizing Agents during Heterologous Overexpression in Escherichia coli – Application to Chlamydial Penicillin-Binding Protein 6

Heterologous overexpression of foreign proteins in Escherichia coli often leads to insoluble aggregates of misfolded inactive proteins, so-called inclusion bodies. To solve this problem use of chaperones or in vitro refolding procedures are the means of choice. These methods are time consuming and c...

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Detalles Bibliográficos
Autores principales: Otten, Christian, De Benedetti, Stefania, Gaballah, Ahmed, Bühl, Henrike, Klöckner, Anna, Brauner, Jarryd, Sahl, Hans-Georg, Henrichfreise, Beate
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388811/
https://www.ncbi.nlm.nih.gov/pubmed/25849314
http://dx.doi.org/10.1371/journal.pone.0122110

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388811/
https://www.ncbi.nlm.nih.gov/pubmed/25849314
http://dx.doi.org/10.1371/journal.pone.0122110

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