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Co-Solvents as Stabilizing Agents during Heterologous Overexpression in Escherichia coli – Application to Chlamydial Penicillin-Binding Protein 6
Heterologous overexpression of foreign proteins in Escherichia coli often leads to insoluble aggregates of misfolded inactive proteins, so-called inclusion bodies. To solve this problem use of chaperones or in vitro refolding procedures are the means of choice. These methods are time consuming and c...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388811/ https://www.ncbi.nlm.nih.gov/pubmed/25849314 http://dx.doi.org/10.1371/journal.pone.0122110 |