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Small Molecule Ice Recrystallization Inhibitors Enable Freezing of Human Red Blood Cells with Reduced Glycerol Concentrations

In North America, red blood cells (RBCs) are cryopreserved in a clinical setting using high glycerol concentrations (40% w/v) with slow cooling rates (~1°C/min) prior to storage at −80°C, while European protocols use reduced glycerol concentrations with rapid freezing rates. After thawing and prior...

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Autores principales: Capicciotti, Chantelle J., Kurach, Jayme D. R., Turner, Tracey R., Mancini, Ross S., Acker, Jason P., Ben, Robert N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389209/
https://www.ncbi.nlm.nih.gov/pubmed/25851700
http://dx.doi.org/10.1038/srep09692
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author Capicciotti, Chantelle J.
Kurach, Jayme D. R.
Turner, Tracey R.
Mancini, Ross S.
Acker, Jason P.
Ben, Robert N.
author_facet Capicciotti, Chantelle J.
Kurach, Jayme D. R.
Turner, Tracey R.
Mancini, Ross S.
Acker, Jason P.
Ben, Robert N.
author_sort Capicciotti, Chantelle J.
collection PubMed
description In North America, red blood cells (RBCs) are cryopreserved in a clinical setting using high glycerol concentrations (40% w/v) with slow cooling rates (~1°C/min) prior to storage at −80°C, while European protocols use reduced glycerol concentrations with rapid freezing rates. After thawing and prior to transfusion, glycerol must be removed to avoid intravascular hemolysis. This is a time consuming process requiring specialized equipment. Small molecule ice recrystallization inhibitors (IRIs) such as β-PMP-Glc and β-pBrPh-Glc have the ability to prevent ice recrystallization, a process that contributes to cellular injury and decreased cell viability after cryopreservation. Herein, we report that addition of 110 mM β-PMP-Glc or 30 mM β-pBrPh-Glc to a 15% glycerol solution increases post-thaw RBC integrity by 30-50% using slow cooling rates and emphasize the potential of small molecule IRIs for the preservation of cells.
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spelling pubmed-43892092015-04-08 Small Molecule Ice Recrystallization Inhibitors Enable Freezing of Human Red Blood Cells with Reduced Glycerol Concentrations Capicciotti, Chantelle J. Kurach, Jayme D. R. Turner, Tracey R. Mancini, Ross S. Acker, Jason P. Ben, Robert N. Sci Rep Article In North America, red blood cells (RBCs) are cryopreserved in a clinical setting using high glycerol concentrations (40% w/v) with slow cooling rates (~1°C/min) prior to storage at −80°C, while European protocols use reduced glycerol concentrations with rapid freezing rates. After thawing and prior to transfusion, glycerol must be removed to avoid intravascular hemolysis. This is a time consuming process requiring specialized equipment. Small molecule ice recrystallization inhibitors (IRIs) such as β-PMP-Glc and β-pBrPh-Glc have the ability to prevent ice recrystallization, a process that contributes to cellular injury and decreased cell viability after cryopreservation. Herein, we report that addition of 110 mM β-PMP-Glc or 30 mM β-pBrPh-Glc to a 15% glycerol solution increases post-thaw RBC integrity by 30-50% using slow cooling rates and emphasize the potential of small molecule IRIs for the preservation of cells. Nature Publishing Group 2015-04-08 /pmc/articles/PMC4389209/ /pubmed/25851700 http://dx.doi.org/10.1038/srep09692 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Capicciotti, Chantelle J.
Kurach, Jayme D. R.
Turner, Tracey R.
Mancini, Ross S.
Acker, Jason P.
Ben, Robert N.
Small Molecule Ice Recrystallization Inhibitors Enable Freezing of Human Red Blood Cells with Reduced Glycerol Concentrations
title Small Molecule Ice Recrystallization Inhibitors Enable Freezing of Human Red Blood Cells with Reduced Glycerol Concentrations
title_full Small Molecule Ice Recrystallization Inhibitors Enable Freezing of Human Red Blood Cells with Reduced Glycerol Concentrations
title_fullStr Small Molecule Ice Recrystallization Inhibitors Enable Freezing of Human Red Blood Cells with Reduced Glycerol Concentrations
title_full_unstemmed Small Molecule Ice Recrystallization Inhibitors Enable Freezing of Human Red Blood Cells with Reduced Glycerol Concentrations
title_short Small Molecule Ice Recrystallization Inhibitors Enable Freezing of Human Red Blood Cells with Reduced Glycerol Concentrations
title_sort small molecule ice recrystallization inhibitors enable freezing of human red blood cells with reduced glycerol concentrations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389209/
https://www.ncbi.nlm.nih.gov/pubmed/25851700
http://dx.doi.org/10.1038/srep09692
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