A rapid and reliable strategy for chromosomal integration of gene(s) with multiple copies

Direct optimization of the metabolic pathways on the chromosome requires tools that can fine tune the overexpression of a desired gene or optimize the combination of multiple genes. Although plasmid-dependent overexpression has been used for this task, fundamental issues concerning its genetic stabi...

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Detalles Bibliográficos
Autores principales: Gu, Pengfei, Yang, Fan, Su, Tianyuan, Wang, Qian, Liang, Quanfeng, Qi, Qingsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389210/
https://www.ncbi.nlm.nih.gov/pubmed/25851494
http://dx.doi.org/10.1038/srep09684

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389210/
https://www.ncbi.nlm.nih.gov/pubmed/25851494
http://dx.doi.org/10.1038/srep09684

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