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Down-regulated miR-331–5p and miR-27a are associated with chemotherapy resistance and relapse in leukaemia
Multidrug resistance (MDR) and disease relapse are challenging clinical problems in the treatment of leukaemia. Relapsed disease is frequently refractory to chemotherapy and exhibits multiple drug resistance. Therefore, it is important to identify the mechanism by which cancer cells develop resistan...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394226/ https://www.ncbi.nlm.nih.gov/pubmed/21070600 http://dx.doi.org/10.1111/j.1582-4934.2010.01213.x |
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author | Feng, Dan-Dan Zhang, Hua Zhang, Peng Zheng, Yu-Sheng Zhang, Xing-Ju Han, Bo-Wei Luo, Xue-Qun Xu, Ling Zhou, Hui Qu, Liang-Hu Chen, Yue-Qin |
author_facet | Feng, Dan-Dan Zhang, Hua Zhang, Peng Zheng, Yu-Sheng Zhang, Xing-Ju Han, Bo-Wei Luo, Xue-Qun Xu, Ling Zhou, Hui Qu, Liang-Hu Chen, Yue-Qin |
author_sort | Feng, Dan-Dan |
collection | PubMed |
description | Multidrug resistance (MDR) and disease relapse are challenging clinical problems in the treatment of leukaemia. Relapsed disease is frequently refractory to chemotherapy and exhibits multiple drug resistance. Therefore, it is important to identify the mechanism by which cancer cells develop resistance. In this study, we used microRNA (miRNA) microarray and qRT-PCR approaches to investigate the expression of miRNAs in three leukaemia cell lines with different degrees of resistance to doxorubicin (DOX) compared with their parent cell line, K562. The expression of miR-331–5p and miR-27a was inversely correlated with the expression of a drug-resistant factor, P-glycoprotein (P-gp), in leukaemia cell lines with gradually increasing resistance. The development of drug resistance is regulated by the expression of the P-gp. Transfection of the K562 and, a human promyelocytic cell line (HL) HL60 DOX-resistant cells with miR-331–5p and miR-27a, separately or in combination, resulted in the increased sensitivity of cells to DOX, suggesting that correction of altered expression of miRNAs may be used for therapeutic strategies to overcome leukaemia cell resistance. Importantly, miR-331–5p and miR-27a were also expressed at lower levels in a panel of relapse patients compared with primary patients at diagnosis, further illustrating that leukaemia relapse might be a consequence of deregulation of miR-331–5p and miR-27a. |
format | Online Article Text |
id | pubmed-4394226 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-43942262015-04-13 Down-regulated miR-331–5p and miR-27a are associated with chemotherapy resistance and relapse in leukaemia Feng, Dan-Dan Zhang, Hua Zhang, Peng Zheng, Yu-Sheng Zhang, Xing-Ju Han, Bo-Wei Luo, Xue-Qun Xu, Ling Zhou, Hui Qu, Liang-Hu Chen, Yue-Qin J Cell Mol Med Articles Multidrug resistance (MDR) and disease relapse are challenging clinical problems in the treatment of leukaemia. Relapsed disease is frequently refractory to chemotherapy and exhibits multiple drug resistance. Therefore, it is important to identify the mechanism by which cancer cells develop resistance. In this study, we used microRNA (miRNA) microarray and qRT-PCR approaches to investigate the expression of miRNAs in three leukaemia cell lines with different degrees of resistance to doxorubicin (DOX) compared with their parent cell line, K562. The expression of miR-331–5p and miR-27a was inversely correlated with the expression of a drug-resistant factor, P-glycoprotein (P-gp), in leukaemia cell lines with gradually increasing resistance. The development of drug resistance is regulated by the expression of the P-gp. Transfection of the K562 and, a human promyelocytic cell line (HL) HL60 DOX-resistant cells with miR-331–5p and miR-27a, separately or in combination, resulted in the increased sensitivity of cells to DOX, suggesting that correction of altered expression of miRNAs may be used for therapeutic strategies to overcome leukaemia cell resistance. Importantly, miR-331–5p and miR-27a were also expressed at lower levels in a panel of relapse patients compared with primary patients at diagnosis, further illustrating that leukaemia relapse might be a consequence of deregulation of miR-331–5p and miR-27a. Blackwell Publishing Ltd 2011-10 2011-09-26 /pmc/articles/PMC4394226/ /pubmed/21070600 http://dx.doi.org/10.1111/j.1582-4934.2010.01213.x Text en © 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd |
spellingShingle | Articles Feng, Dan-Dan Zhang, Hua Zhang, Peng Zheng, Yu-Sheng Zhang, Xing-Ju Han, Bo-Wei Luo, Xue-Qun Xu, Ling Zhou, Hui Qu, Liang-Hu Chen, Yue-Qin Down-regulated miR-331–5p and miR-27a are associated with chemotherapy resistance and relapse in leukaemia |
title | Down-regulated miR-331–5p and miR-27a are associated with chemotherapy resistance and relapse in leukaemia |
title_full | Down-regulated miR-331–5p and miR-27a are associated with chemotherapy resistance and relapse in leukaemia |
title_fullStr | Down-regulated miR-331–5p and miR-27a are associated with chemotherapy resistance and relapse in leukaemia |
title_full_unstemmed | Down-regulated miR-331–5p and miR-27a are associated with chemotherapy resistance and relapse in leukaemia |
title_short | Down-regulated miR-331–5p and miR-27a are associated with chemotherapy resistance and relapse in leukaemia |
title_sort | down-regulated mir-331–5p and mir-27a are associated with chemotherapy resistance and relapse in leukaemia |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394226/ https://www.ncbi.nlm.nih.gov/pubmed/21070600 http://dx.doi.org/10.1111/j.1582-4934.2010.01213.x |
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