Monitoring of Apoptosis in 3D Cell Cultures by FRET and Light Sheet Fluorescence Microscopy

Non-radiative cell membrane associated Förster Resonance Energy Transfer (FRET) from an enhanced cyan fluorescent protein (ECFP) to an enhanced yellow fluorescent protein (EYFP) is used for detection of apoptosis in 3-dimensional cell cultures. FRET is visualized in multi-cellular tumor spheroids by...

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Detalles Bibliográficos
Autores principales: Weber, Petra, Schickinger, Sarah, Wagner, Michael, Angres, Brigitte, Bruns, Thomas, Schneckenburger, Herbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394481/
https://www.ncbi.nlm.nih.gov/pubmed/25761242
http://dx.doi.org/10.3390/ijms16035375

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394481/
https://www.ncbi.nlm.nih.gov/pubmed/25761242
http://dx.doi.org/10.3390/ijms16035375

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