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G3BP1 promotes stress-induced RNA granule interactions to preserve polyadenylated mRNA

G3BP1, a target of TDP-43, is required for normal stress granule (SG) assembly, but the functional consequences of failed SG assembly remain unknown. Here, using both transformed cell lines and primary neurons, we investigated the functional impact of this disruption in SG dynamics. While stress-ind...

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Autores principales: Aulas, Anaïs, Caron, Guillaume, Gkogkas, Christos G., Mohamed, Nguyen-Vi, Destroismaisons, Laurie, Sonenberg, Nahum, Leclerc, Nicole, Parker, J. Alex, Vande Velde, Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395486/
https://www.ncbi.nlm.nih.gov/pubmed/25847539
http://dx.doi.org/10.1083/jcb.201408092
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author Aulas, Anaïs
Caron, Guillaume
Gkogkas, Christos G.
Mohamed, Nguyen-Vi
Destroismaisons, Laurie
Sonenberg, Nahum
Leclerc, Nicole
Parker, J. Alex
Vande Velde, Christine
author_facet Aulas, Anaïs
Caron, Guillaume
Gkogkas, Christos G.
Mohamed, Nguyen-Vi
Destroismaisons, Laurie
Sonenberg, Nahum
Leclerc, Nicole
Parker, J. Alex
Vande Velde, Christine
author_sort Aulas, Anaïs
collection PubMed
description G3BP1, a target of TDP-43, is required for normal stress granule (SG) assembly, but the functional consequences of failed SG assembly remain unknown. Here, using both transformed cell lines and primary neurons, we investigated the functional impact of this disruption in SG dynamics. While stress-induced translational repression and recruitment of key SG proteins was undisturbed, depletion of G3BP1 or its upstream regulator TDP-43 disturbed normal interactions between SGs and processing bodies (PBs). This was concomitant with decreased SG size, reduced SG–PB docking, and impaired preservation of polyadenylated mRNA. Reintroduction of G3BP1 alone was sufficient to rescue all of these phenotypes, indicating that G3BP1 is essential for normal SG–PB interactions and SG function.
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spelling pubmed-43954862015-10-13 G3BP1 promotes stress-induced RNA granule interactions to preserve polyadenylated mRNA Aulas, Anaïs Caron, Guillaume Gkogkas, Christos G. Mohamed, Nguyen-Vi Destroismaisons, Laurie Sonenberg, Nahum Leclerc, Nicole Parker, J. Alex Vande Velde, Christine J Cell Biol Research Articles G3BP1, a target of TDP-43, is required for normal stress granule (SG) assembly, but the functional consequences of failed SG assembly remain unknown. Here, using both transformed cell lines and primary neurons, we investigated the functional impact of this disruption in SG dynamics. While stress-induced translational repression and recruitment of key SG proteins was undisturbed, depletion of G3BP1 or its upstream regulator TDP-43 disturbed normal interactions between SGs and processing bodies (PBs). This was concomitant with decreased SG size, reduced SG–PB docking, and impaired preservation of polyadenylated mRNA. Reintroduction of G3BP1 alone was sufficient to rescue all of these phenotypes, indicating that G3BP1 is essential for normal SG–PB interactions and SG function. The Rockefeller University Press 2015-04-13 /pmc/articles/PMC4395486/ /pubmed/25847539 http://dx.doi.org/10.1083/jcb.201408092 Text en © 2015 Aulas et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Aulas, Anaïs
Caron, Guillaume
Gkogkas, Christos G.
Mohamed, Nguyen-Vi
Destroismaisons, Laurie
Sonenberg, Nahum
Leclerc, Nicole
Parker, J. Alex
Vande Velde, Christine
G3BP1 promotes stress-induced RNA granule interactions to preserve polyadenylated mRNA
title G3BP1 promotes stress-induced RNA granule interactions to preserve polyadenylated mRNA
title_full G3BP1 promotes stress-induced RNA granule interactions to preserve polyadenylated mRNA
title_fullStr G3BP1 promotes stress-induced RNA granule interactions to preserve polyadenylated mRNA
title_full_unstemmed G3BP1 promotes stress-induced RNA granule interactions to preserve polyadenylated mRNA
title_short G3BP1 promotes stress-induced RNA granule interactions to preserve polyadenylated mRNA
title_sort g3bp1 promotes stress-induced rna granule interactions to preserve polyadenylated mrna
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395486/
https://www.ncbi.nlm.nih.gov/pubmed/25847539
http://dx.doi.org/10.1083/jcb.201408092
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