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O-Glycosylation in Cell Wall Proteins in Scedosporium prolificans Is Critical for Phagocytosis and Inflammatory Cytokines Production by Macrophages

In this study, we analyze the importance of O-linked oligosaccharides present in peptidorhamnomannan (PRM) from the cell wall of the fungus Scedosporium prolificans for recognition and phagocytosis of conidia by macrophages. Adding PRM led to a dose-dependent inhibition of conidia phagocytosis, wher...

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Autores principales: Xisto, Mariana I. D. S., Bittencourt, Vera C. B., Liporagi-Lopes, Livia Cristina, Haido, Rosa M. T., Mendonça, Morena S. A., Sassaki, Guilherme, Figueiredo, Rodrigo T., Romanos, Maria Teresa V., Barreto-Bergter, Eliana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396840/
https://www.ncbi.nlm.nih.gov/pubmed/25875427
http://dx.doi.org/10.1371/journal.pone.0123189
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author Xisto, Mariana I. D. S.
Bittencourt, Vera C. B.
Liporagi-Lopes, Livia Cristina
Haido, Rosa M. T.
Mendonça, Morena S. A.
Sassaki, Guilherme
Figueiredo, Rodrigo T.
Romanos, Maria Teresa V.
Barreto-Bergter, Eliana
author_facet Xisto, Mariana I. D. S.
Bittencourt, Vera C. B.
Liporagi-Lopes, Livia Cristina
Haido, Rosa M. T.
Mendonça, Morena S. A.
Sassaki, Guilherme
Figueiredo, Rodrigo T.
Romanos, Maria Teresa V.
Barreto-Bergter, Eliana
author_sort Xisto, Mariana I. D. S.
collection PubMed
description In this study, we analyze the importance of O-linked oligosaccharides present in peptidorhamnomannan (PRM) from the cell wall of the fungus Scedosporium prolificans for recognition and phagocytosis of conidia by macrophages. Adding PRM led to a dose-dependent inhibition of conidia phagocytosis, whereas de-O-glycosylated PRM did not show any effect. PRM induced the release of macrophage-derived antimicrobial compounds. However, O-linked oligosaccharides do not appear to be required for such induction. The effect of PRM on conidia-induced macrophage killing was examined using latex beads coated with PRM or de-O-glycosylated PRM. A decrease in macrophage viability similar to that caused by conidia was detected. However, macrophage killing was unaffected when beads coated with de-O-glycosylated PRM were used, indicating the toxic effect of O-linked oligosaccharides on macrophages. In addition, PRM triggered TNF-α release by macrophages. Chemical removal of O-linked oligosaccharides from PRM abolished cytokine induction, suggesting that the O-linked oligosaccharidic chains are important moieties involved in inflammatory responses through the induction of TNF-α secretion. In summary, we show that O-glycosylation plays a role in the recognition and uptake of S. prolificans by macrophages, killing of macrophages and production of pro- inflammatory cytokines.
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spelling pubmed-43968402015-04-21 O-Glycosylation in Cell Wall Proteins in Scedosporium prolificans Is Critical for Phagocytosis and Inflammatory Cytokines Production by Macrophages Xisto, Mariana I. D. S. Bittencourt, Vera C. B. Liporagi-Lopes, Livia Cristina Haido, Rosa M. T. Mendonça, Morena S. A. Sassaki, Guilherme Figueiredo, Rodrigo T. Romanos, Maria Teresa V. Barreto-Bergter, Eliana PLoS One Research Article In this study, we analyze the importance of O-linked oligosaccharides present in peptidorhamnomannan (PRM) from the cell wall of the fungus Scedosporium prolificans for recognition and phagocytosis of conidia by macrophages. Adding PRM led to a dose-dependent inhibition of conidia phagocytosis, whereas de-O-glycosylated PRM did not show any effect. PRM induced the release of macrophage-derived antimicrobial compounds. However, O-linked oligosaccharides do not appear to be required for such induction. The effect of PRM on conidia-induced macrophage killing was examined using latex beads coated with PRM or de-O-glycosylated PRM. A decrease in macrophage viability similar to that caused by conidia was detected. However, macrophage killing was unaffected when beads coated with de-O-glycosylated PRM were used, indicating the toxic effect of O-linked oligosaccharides on macrophages. In addition, PRM triggered TNF-α release by macrophages. Chemical removal of O-linked oligosaccharides from PRM abolished cytokine induction, suggesting that the O-linked oligosaccharidic chains are important moieties involved in inflammatory responses through the induction of TNF-α secretion. In summary, we show that O-glycosylation plays a role in the recognition and uptake of S. prolificans by macrophages, killing of macrophages and production of pro- inflammatory cytokines. Public Library of Science 2015-04-14 /pmc/articles/PMC4396840/ /pubmed/25875427 http://dx.doi.org/10.1371/journal.pone.0123189 Text en © 2015 Xisto et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Xisto, Mariana I. D. S.
Bittencourt, Vera C. B.
Liporagi-Lopes, Livia Cristina
Haido, Rosa M. T.
Mendonça, Morena S. A.
Sassaki, Guilherme
Figueiredo, Rodrigo T.
Romanos, Maria Teresa V.
Barreto-Bergter, Eliana
O-Glycosylation in Cell Wall Proteins in Scedosporium prolificans Is Critical for Phagocytosis and Inflammatory Cytokines Production by Macrophages
title O-Glycosylation in Cell Wall Proteins in Scedosporium prolificans Is Critical for Phagocytosis and Inflammatory Cytokines Production by Macrophages
title_full O-Glycosylation in Cell Wall Proteins in Scedosporium prolificans Is Critical for Phagocytosis and Inflammatory Cytokines Production by Macrophages
title_fullStr O-Glycosylation in Cell Wall Proteins in Scedosporium prolificans Is Critical for Phagocytosis and Inflammatory Cytokines Production by Macrophages
title_full_unstemmed O-Glycosylation in Cell Wall Proteins in Scedosporium prolificans Is Critical for Phagocytosis and Inflammatory Cytokines Production by Macrophages
title_short O-Glycosylation in Cell Wall Proteins in Scedosporium prolificans Is Critical for Phagocytosis and Inflammatory Cytokines Production by Macrophages
title_sort o-glycosylation in cell wall proteins in scedosporium prolificans is critical for phagocytosis and inflammatory cytokines production by macrophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396840/
https://www.ncbi.nlm.nih.gov/pubmed/25875427
http://dx.doi.org/10.1371/journal.pone.0123189
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