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A Simple and Robust Method for Establishing Homogeneous Mouse Epiblast Stem Cell Lines by Wnt Inhibition

Epiblast stem cells (EpiSCs) are pluripotent stem cells derived from epiblasts of postimplantation mouse embryos, and thus provide a useful model for studying “primed” pluripotent states. Here, we devised a simple and robust technique to derive high-quality EpiSCs using an inhibitor of WNT secretion...

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Detalles Bibliográficos
Autores principales: Sugimoto, Michihiko, Kondo, Masayo, Koga, Yumiko, Shiura, Hirosuke, Ikeda, Rieko, Hirose, Michiko, Ogura, Atsuo, Murakami, Ayumi, Yoshiki, Atsushi, Chuva de Sousa Lopes, Susana M., Abe, Kuniya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4400648/
https://www.ncbi.nlm.nih.gov/pubmed/25818811
http://dx.doi.org/10.1016/j.stemcr.2015.02.014