Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Proteins are the building blocks, effectors and signal mediators of cellular processes. A protein’s function, regulation and localization often depend on its interactions with other proteins. Here, we describe a protocol for the yeast protein-fragment complementation assay (PCA), a powerful method t...

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Detalles Bibliográficos
Autores principales: Rochette, Samuel, Diss, Guillaume, Filteau, Marie, Leducq, Jean-Baptiste, Dubé, Alexandre K., Landry, Christian R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Cellular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401175/
https://www.ncbi.nlm.nih.gov/pubmed/25867901
http://dx.doi.org/10.3791/52255

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401175/
https://www.ncbi.nlm.nih.gov/pubmed/25867901
http://dx.doi.org/10.3791/52255

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