Cargando…

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Proteins are the building blocks, effectors and signal mediators of cellular processes. A protein’s function, regulation and localization often depend on its interactions with other proteins. Here, we describe a protocol for the yeast protein-fragment complementation assay (PCA), a powerful method t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Rochette, Samuel, Diss, Guillaume, Filteau, Marie, Leducq, Jean-Baptiste, Dubé, Alexandre K., Landry, Christian R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2015
Materias:	Cellular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401175/ https://www.ncbi.nlm.nih.gov/pubmed/25867901 http://dx.doi.org/10.3791/52255

Ejemplares similares

Evidence for the Robustness of Protein Complexes to Inter-Species Hybridization
por: Leducq, Jean-Baptiste, et al.
Publicado: (2012)

Barcode fusion genetics-protein-fragment complementation assay (BFG-PCA): tools and resources that expand the potential for binary protein interaction discovery
por: Evans-Yamamoto, Daniel, et al.
Publicado: (2022)

Protein-fragment complementation assays for large-scale analysis of protein–protein interactions
por: Blaszczak, Ewa, et al.
Publicado: (2021)

Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments
por: Ohmuro-Matsuyama, Yuki, et al.
Publicado: (2013)

Live-cell monitoring of protein localization to membrane rafts using protein-fragment complementation
por: Merezhko, Maria, et al.
Publicado: (2020)

A Live Cell Protein Complementation Assay for ORFeome-Wide Probing of Human HOX Interactomes
por: Jia, Yunlong, et al.
Publicado: (2023)

Optimizing the fragment complementation of APEX2 for detection of specific protein-protein interactions in live cells
por: Xue, Miaomiao, et al.
Publicado: (2017)

Author Correction: Optimizing the fragment complementation of APEX2 for detection of specific protein-protein interactions in live cells
por: Xue, Miaomiao, et al.
Publicado: (2018)

Publisher Correction: Optimizing the fragment complementation of APEX2 for detection of specific protein-protein interactions in live cells
por: Xue, Miaomiao, et al.
Publicado: (2018)

Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay
por: Hudry, Bruno, et al.
Publicado: (2011)

GFP-complementation assay to detect functional CPP and protein delivery into living cells
por: Milech, Nadia, et al.
Publicado: (2015)

Optimization of RiPCA for the Live‐Cell Detection of Pre‐MicroRNA‐Protein Interactions
por: Rosenblum, Sydney L., et al.
Publicado: (2022)

4D Imaging of Protein Aggregation in Live Cells
por: Spokoini, Rachel, et al.
Publicado: (2013)

A new location to split Cre recombinase for protein fragment complementation
por: Rajaee, Maryam, et al.
Publicado: (2017)

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
por: Caers, Jelle, et al.
Publicado: (2014)

A comparison of tissue-based and recombinant protein–based assays for detecting PCA-Tr/DNER-IgG
por: Alfugham, Nora, et al.
Publicado: (2016)

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
por: Kremers, Gert-Jan, et al.
Publicado: (2010)

The PCA and LDA Analysis on the Differential Expression of Proteins in Breast Cancer
por: Liang, Seng, et al.
Publicado: (2010)

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
por: Konig, Julian, et al.
Publicado: (2011)

Imaging Protein-protein Interactions in vivo
por: Seegar, Tom, et al.
Publicado: (2010)

The complement system of the goat: Haemolytic assays and isolation of major proteins
por: Moreno-Indias, Isabel, et al.
Publicado: (2012)

Expression attenuation as a mechanism of robustness against gene duplication
por: Ascencio, Diana, et al.
Publicado: (2021)

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
por: Hafner, Markus, et al.
Publicado: (2010)

Secretion of soluble complement inhibitors factor H and factor H-like protein (FHL-1) by ovarian tumour cells
por: Junnikkala, S, et al.
Publicado: (2002)

Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging
por: Malinovska, Liliana, et al.
Publicado: (2016)

Combining intracellular selection with protein-fragment complementation to derive Aβ interacting peptides
por: Acerra, Nicola, et al.
Publicado: (2013)

Establishment of tetracycline-regulated bimolecular fluorescence complementation assay to detect protein-protein interactions in Candida albicans
por: Lai, Wei-Chung, et al.
Publicado: (2020)

Ixodes scapularis nymph saliva protein blocks host inflammation and complement-mediated killing of Lyme disease agent, Borrelia burgdorferi
por: Bencosme-Cuevas, Emily, et al.
Publicado: (2023)

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
por: Sinclair, Maureen, et al.
Publicado: (2012)

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
por: Daniels, Danette L., et al.
Publicado: (2014)

Enzyme-Linked Aptamer Assay (ELAA) for Detection of Toxoplasma ROP18 Protein in Human Serum
por: Vargas-Montes, Monica, et al.
Publicado: (2019)

Where Do Phosphosites Come from and Where Do They Go after Gene Duplication?
por: Diss, Guillaume, et al.
Publicado: (2012)

The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles
por: Dallastella, Marianna, et al.
Publicado: (2023)

Specificity Protein 1: A Protein With a Two-Sided Role in Ischemic Stroke
por: Yu, Qinyang, et al.
Publicado: (2021)

Vectors for multi-color bimolecular fluorescence complementation to investigate protein-protein interactions in living plant cells
por: Lee, Lan-Ying, et al.
Publicado: (2008)

Engineering Cell-permeable Protein
por: Münst, Bernhard, et al.
Publicado: (2009)

Interaction between Ku80 protein and a widely used antibody to adenomatous polyposis coli
por: Roberts, G T, et al.
Publicado: (2003)

Editorial: Complement in nervous system disease
por: Michailidou, Iliana, et al.
Publicado: (2023)

Split luciferase complementation assay to detect regulated protein-protein interactions in rice protoplasts in a large-scale format
por: Fujikawa, Yukichi, et al.
Publicado: (2014)

Phosphorylation-Assisted Luciferase Complementation Assay Designed to Monitor Kinase Activity and Kinase-Domain-Mediated Protein–Protein Binding
por: Póti, Ádám L., et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_oicb2ppr3jbv0kjna5v046hicg