Cargando…
Persistence of Smoking-Induced Dysregulation of MiRNA Expression in the Small Airway Epithelium Despite Smoking Cessation
Even after quitting smoking, the risk of the development of chronic obstructive pulmonary disease (COPD) and lung cancer remains significantly higher compared to healthy nonsmokers. Based on the knowledge that COPD and most lung cancers start in the small airway epithelium (SAE), we hypothesized tha...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401720/ https://www.ncbi.nlm.nih.gov/pubmed/25886353 http://dx.doi.org/10.1371/journal.pone.0120824 |
_version_ | 1782367180960038912 |
---|---|
author | Wang, Guoqing Wang, Rui Strulovici-Barel, Yael Salit, Jacqueline Staudt, Michelle R. Ahmed, Joumana Tilley, Ann E. Yee-Levin, Jenny Hollmann, Charleen Harvey, Ben-Gary Kaner, Robert J. Mezey, Jason G. Sridhar, Sriram Pillai, Sreekumar G. Hilton, Holly Wolff, Gerhard Bitter, Hans Visvanathan, Sudha Fine, Jay S. Stevenson, Christopher S. Crystal, Ronald G. |
author_facet | Wang, Guoqing Wang, Rui Strulovici-Barel, Yael Salit, Jacqueline Staudt, Michelle R. Ahmed, Joumana Tilley, Ann E. Yee-Levin, Jenny Hollmann, Charleen Harvey, Ben-Gary Kaner, Robert J. Mezey, Jason G. Sridhar, Sriram Pillai, Sreekumar G. Hilton, Holly Wolff, Gerhard Bitter, Hans Visvanathan, Sudha Fine, Jay S. Stevenson, Christopher S. Crystal, Ronald G. |
author_sort | Wang, Guoqing |
collection | PubMed |
description | Even after quitting smoking, the risk of the development of chronic obstructive pulmonary disease (COPD) and lung cancer remains significantly higher compared to healthy nonsmokers. Based on the knowledge that COPD and most lung cancers start in the small airway epithelium (SAE), we hypothesized that smoking modulates miRNA expression in the SAE linked to the pathogenesis of smoking-induced airway disease, and that some of these changes persist after smoking cessation. SAE was collected from 10(th) to 12(th) order bronchi using fiberoptic bronchoscopy. Affymetrix miRNA 2.0 arrays were used to assess miRNA expression in the SAE from 9 healthy nonsmokers and 10 healthy smokers, before and after they quit smoking for 3 months. Smoking status was determined by urine nicotine and cotinine measurement. There were significant differences in the expression of 34 miRNAs between healthy smokers and healthy nonsmokers (p<0.01, fold-change >1.5), with functions associated with lung development, airway epithelium differentiation, inflammation and cancer. After quitting smoking for 3 months, 12 out of the 34 miRNAs did not return to normal levels, with Wnt/β-catenin signaling pathway being the top identified enriched pathway of the target genes of the persistent dysregulated miRNAs. In the context that many of these persistent smoking-dependent miRNAs are associated with differentiation, inflammatory diseases or lung cancer, it is likely that persistent smoking-related changes in SAE miRNAs play a role in the subsequent development of these disorders. |
format | Online Article Text |
id | pubmed-4401720 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44017202015-04-21 Persistence of Smoking-Induced Dysregulation of MiRNA Expression in the Small Airway Epithelium Despite Smoking Cessation Wang, Guoqing Wang, Rui Strulovici-Barel, Yael Salit, Jacqueline Staudt, Michelle R. Ahmed, Joumana Tilley, Ann E. Yee-Levin, Jenny Hollmann, Charleen Harvey, Ben-Gary Kaner, Robert J. Mezey, Jason G. Sridhar, Sriram Pillai, Sreekumar G. Hilton, Holly Wolff, Gerhard Bitter, Hans Visvanathan, Sudha Fine, Jay S. Stevenson, Christopher S. Crystal, Ronald G. PLoS One Research Article Even after quitting smoking, the risk of the development of chronic obstructive pulmonary disease (COPD) and lung cancer remains significantly higher compared to healthy nonsmokers. Based on the knowledge that COPD and most lung cancers start in the small airway epithelium (SAE), we hypothesized that smoking modulates miRNA expression in the SAE linked to the pathogenesis of smoking-induced airway disease, and that some of these changes persist after smoking cessation. SAE was collected from 10(th) to 12(th) order bronchi using fiberoptic bronchoscopy. Affymetrix miRNA 2.0 arrays were used to assess miRNA expression in the SAE from 9 healthy nonsmokers and 10 healthy smokers, before and after they quit smoking for 3 months. Smoking status was determined by urine nicotine and cotinine measurement. There were significant differences in the expression of 34 miRNAs between healthy smokers and healthy nonsmokers (p<0.01, fold-change >1.5), with functions associated with lung development, airway epithelium differentiation, inflammation and cancer. After quitting smoking for 3 months, 12 out of the 34 miRNAs did not return to normal levels, with Wnt/β-catenin signaling pathway being the top identified enriched pathway of the target genes of the persistent dysregulated miRNAs. In the context that many of these persistent smoking-dependent miRNAs are associated with differentiation, inflammatory diseases or lung cancer, it is likely that persistent smoking-related changes in SAE miRNAs play a role in the subsequent development of these disorders. Public Library of Science 2015-04-17 /pmc/articles/PMC4401720/ /pubmed/25886353 http://dx.doi.org/10.1371/journal.pone.0120824 Text en © 2015 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wang, Guoqing Wang, Rui Strulovici-Barel, Yael Salit, Jacqueline Staudt, Michelle R. Ahmed, Joumana Tilley, Ann E. Yee-Levin, Jenny Hollmann, Charleen Harvey, Ben-Gary Kaner, Robert J. Mezey, Jason G. Sridhar, Sriram Pillai, Sreekumar G. Hilton, Holly Wolff, Gerhard Bitter, Hans Visvanathan, Sudha Fine, Jay S. Stevenson, Christopher S. Crystal, Ronald G. Persistence of Smoking-Induced Dysregulation of MiRNA Expression in the Small Airway Epithelium Despite Smoking Cessation |
title | Persistence of Smoking-Induced Dysregulation of MiRNA Expression in the Small Airway Epithelium Despite Smoking Cessation |
title_full | Persistence of Smoking-Induced Dysregulation of MiRNA Expression in the Small Airway Epithelium Despite Smoking Cessation |
title_fullStr | Persistence of Smoking-Induced Dysregulation of MiRNA Expression in the Small Airway Epithelium Despite Smoking Cessation |
title_full_unstemmed | Persistence of Smoking-Induced Dysregulation of MiRNA Expression in the Small Airway Epithelium Despite Smoking Cessation |
title_short | Persistence of Smoking-Induced Dysregulation of MiRNA Expression in the Small Airway Epithelium Despite Smoking Cessation |
title_sort | persistence of smoking-induced dysregulation of mirna expression in the small airway epithelium despite smoking cessation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401720/ https://www.ncbi.nlm.nih.gov/pubmed/25886353 http://dx.doi.org/10.1371/journal.pone.0120824 |
work_keys_str_mv | AT wangguoqing persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT wangrui persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT strulovicibarelyael persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT salitjacqueline persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT staudtmicheller persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT ahmedjoumana persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT tilleyanne persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT yeelevinjenny persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT hollmanncharleen persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT harveybengary persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT kanerrobertj persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT mezeyjasong persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT sridharsriram persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT pillaisreekumarg persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT hiltonholly persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT wolffgerhard persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT bitterhans persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT visvanathansudha persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT finejays persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT stevensonchristophers persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation AT crystalronaldg persistenceofsmokinginduceddysregulationofmirnaexpressioninthesmallairwayepitheliumdespitesmokingcessation |