A robust assay to measure DNA topology-dependent protein binding affinity

DNA structure and topology pervasively influence aspects of DNA metabolism including replication, transcription and segregation. However, the effects of DNA topology on DNA-protein interactions have not been systematically explored due to limitations of standard affinity assays. We developed a metho...

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Detalles Bibliográficos
Autores principales: Litwin, Tamara R., Solà, Maria, Holt, Ian J., Neuman, Keir C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402506/
https://www.ncbi.nlm.nih.gov/pubmed/25552413
http://dx.doi.org/10.1093/nar/gku1381

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402506/
https://www.ncbi.nlm.nih.gov/pubmed/25552413
http://dx.doi.org/10.1093/nar/gku1381

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