Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi

Gene knockout strategies, RNAi and rescue experiments are all employed to study mammalian gene function. However, the disadvantages of these approaches include: loss of function adaptation, reduced viability and gene overexpression that rarely matches endogenous levels. Here, we developed an endogen...

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Detalles Bibliográficos
Autores principales: Kaulich, Manuel, Lee, Yeon J., Lönn, Peter, Springer, Aaron D., Meade, Bryan R., Dowdy, Steven F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402508/
https://www.ncbi.nlm.nih.gov/pubmed/25586224
http://dx.doi.org/10.1093/nar/gku1403

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402508/
https://www.ncbi.nlm.nih.gov/pubmed/25586224
http://dx.doi.org/10.1093/nar/gku1403

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