Solid-phase cloning for high-throughput assembly of single and multiple DNA parts

We describe solid-phase cloning (SPC) for high-throughput assembly of expression plasmids. Our method allows PCR products to be put directly into a liquid handler for capture and purification using paramagnetic streptavidin beads and conversion into constructs by subsequent cloning reactions. We pre...

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Detalles Bibliográficos
Autores principales: Lundqvist, Magnus, Edfors, Fredrik, Sivertsson, Åsa, Hallström, Björn M., Hudson, Elton P., Tegel, Hanna, Holmberg, Anders, Uhlén, Mathias, Rockberg, Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402512/
https://www.ncbi.nlm.nih.gov/pubmed/25618848
http://dx.doi.org/10.1093/nar/gkv036

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402512/
https://www.ncbi.nlm.nih.gov/pubmed/25618848
http://dx.doi.org/10.1093/nar/gkv036

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