A general approach to high-yield biosynthesis of chimeric RNAs bearing various types of functional small RNAs for broad applications

RNA research and therapy relies primarily on synthetic RNAs. We employed recombinant RNA technology toward large-scale production of pre-miRNA agents in bacteria, but found the majority of target RNAs were not or negligibly expressed. We thus developed a novel strategy to achieve consistent high-yie...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Qiu-Xia, Wang, Wei-Peng, Zeng, Su, Urayama, Shiro, Yu, Ai-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402540/
https://www.ncbi.nlm.nih.gov/pubmed/25800741
http://dx.doi.org/10.1093/nar/gkv228

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402540/
https://www.ncbi.nlm.nih.gov/pubmed/25800741
http://dx.doi.org/10.1093/nar/gkv228

Ejemplares similares