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CRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells

Overexpression is a notorious concern in conventional and especially in super-resolution fluorescence light microscopy studies because it may cause numerous artifacts including ectopic sub-cellular localizations, erroneous formation of protein complexes, and others. Nonetheless, current live cell su...

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Detalles Bibliográficos
Autores principales: Ratz, Michael, Testa, Ilaria, Hell, Stefan W., Jakobs, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402611/
https://www.ncbi.nlm.nih.gov/pubmed/25892259
http://dx.doi.org/10.1038/srep09592