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CRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells
Overexpression is a notorious concern in conventional and especially in super-resolution fluorescence light microscopy studies because it may cause numerous artifacts including ectopic sub-cellular localizations, erroneous formation of protein complexes, and others. Nonetheless, current live cell su...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402611/ https://www.ncbi.nlm.nih.gov/pubmed/25892259 http://dx.doi.org/10.1038/srep09592 |