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Enhancing Protein Expression in HEK-293 Cells by Lowering Culture Temperature
Animal cells and cell lines, such as HEK-293 cells, are commonly cultured at 37°C. These cells are often used to express recombinant proteins. Having a higher expression level or a higher protein yield is generally desirable. As we demonstrate in this study, dropping culture temperature to 33°C, but...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4404257/ https://www.ncbi.nlm.nih.gov/pubmed/25893827 http://dx.doi.org/10.1371/journal.pone.0123562 |