Inducible and combinatorial gene manipulation in mouse brain

We have deployed recombinant adeno-associated viruses equipped with tetracycline-controlled genetic switches to manipulate gene expression in mouse brain. Here, we show a combinatorial genetic approach for inducible, cell type-specific gene expression and Cre/loxP mediated gene recombination in diff...

Descripción completa

Detalles Bibliográficos
Autores principales: Dogbevia, Godwin K., Marticorena-Alvarez, Ricardo, Bausen, Melanie, Sprengel, Rolf, Hasan, Mazahir T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4404871/
https://www.ncbi.nlm.nih.gov/pubmed/25954155
http://dx.doi.org/10.3389/fncel.2015.00142
_version_ 1782367562530553856
author Dogbevia, Godwin K.
Marticorena-Alvarez, Ricardo
Bausen, Melanie
Sprengel, Rolf
Hasan, Mazahir T.
author_facet Dogbevia, Godwin K.
Marticorena-Alvarez, Ricardo
Bausen, Melanie
Sprengel, Rolf
Hasan, Mazahir T.
author_sort Dogbevia, Godwin K.
collection PubMed
description We have deployed recombinant adeno-associated viruses equipped with tetracycline-controlled genetic switches to manipulate gene expression in mouse brain. Here, we show a combinatorial genetic approach for inducible, cell type-specific gene expression and Cre/loxP mediated gene recombination in different brain regions. Our chemical-genetic approach will help to investigate ‘when’, ‘where’, and ‘how’ gene(s) control neuronal circuit dynamics, and organize, for example, sensory signal processing, learning and memory, and behavior.
format Online
Article
Text
id pubmed-4404871
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-44048712015-05-07 Inducible and combinatorial gene manipulation in mouse brain Dogbevia, Godwin K. Marticorena-Alvarez, Ricardo Bausen, Melanie Sprengel, Rolf Hasan, Mazahir T. Front Cell Neurosci Neuroscience We have deployed recombinant adeno-associated viruses equipped with tetracycline-controlled genetic switches to manipulate gene expression in mouse brain. Here, we show a combinatorial genetic approach for inducible, cell type-specific gene expression and Cre/loxP mediated gene recombination in different brain regions. Our chemical-genetic approach will help to investigate ‘when’, ‘where’, and ‘how’ gene(s) control neuronal circuit dynamics, and organize, for example, sensory signal processing, learning and memory, and behavior. Frontiers Media S.A. 2015-04-21 /pmc/articles/PMC4404871/ /pubmed/25954155 http://dx.doi.org/10.3389/fncel.2015.00142 Text en Copyright © 2015 Dogbevia, Marticorena-Alvarez, Bausen, Sprengel and Hasan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Dogbevia, Godwin K.
Marticorena-Alvarez, Ricardo
Bausen, Melanie
Sprengel, Rolf
Hasan, Mazahir T.
Inducible and combinatorial gene manipulation in mouse brain
title Inducible and combinatorial gene manipulation in mouse brain
title_full Inducible and combinatorial gene manipulation in mouse brain
title_fullStr Inducible and combinatorial gene manipulation in mouse brain
title_full_unstemmed Inducible and combinatorial gene manipulation in mouse brain
title_short Inducible and combinatorial gene manipulation in mouse brain
title_sort inducible and combinatorial gene manipulation in mouse brain
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4404871/
https://www.ncbi.nlm.nih.gov/pubmed/25954155
http://dx.doi.org/10.3389/fncel.2015.00142
work_keys_str_mv AT dogbeviagodwink inducibleandcombinatorialgenemanipulationinmousebrain
AT marticorenaalvarezricardo inducibleandcombinatorialgenemanipulationinmousebrain
AT bausenmelanie inducibleandcombinatorialgenemanipulationinmousebrain
AT sprengelrolf inducibleandcombinatorialgenemanipulationinmousebrain
AT hasanmazahirt inducibleandcombinatorialgenemanipulationinmousebrain

Ejemplares similares