Thermal Stabilization of Dihydrofolate Reductase Using Monte Carlo Unfolding Simulations and Its Functional Consequences

Design of proteins with desired thermal properties is important for scientific and biotechnological applications. Here we developed a theoretical approach to predict the effect of mutations on protein stability from non-equilibrium unfolding simulations. We establish a relative measure based on appa...

Descripción completa

Detalles Bibliográficos
Autores principales: Tian, Jian, Woodard, Jaie C., Whitney, Andrew, Shakhnovich, Eugene I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4407897/
https://www.ncbi.nlm.nih.gov/pubmed/25905910
http://dx.doi.org/10.1371/journal.pcbi.1004207

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4407897/
https://www.ncbi.nlm.nih.gov/pubmed/25905910
http://dx.doi.org/10.1371/journal.pcbi.1004207

Ejemplares similares