Use of Stopped-Flow Fluorescence and Labeled Nucleotides to Analyze the ATP Turnover Cycle of Kinesins

The kinesin superfamily of microtubule associated motor proteins share a characteristic motor domain which both hydrolyses ATP and binds microtubules. Kinesins display differences across the superfamily both in ATP turnover and in microtubule interaction. These differences tailor specific kinesins t...

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Detalles Bibliográficos
Autores principales: Patel, Jennifer T., Belsham, Hannah R., Rathbone, Alexandra J., Friel, Claire T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2014
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408023/
https://www.ncbi.nlm.nih.gov/pubmed/25350116
http://dx.doi.org/10.3791/52142

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408023/
https://www.ncbi.nlm.nih.gov/pubmed/25350116
http://dx.doi.org/10.3791/52142

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