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Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull
Large macromolecular complexes such as the spliceosomal small nuclear ribonucleoproteins (snRNPs) play a variety of roles within the cell. Despite their biological importance, biochemical studies of snRNPs and other machines are often thwarted by practical difficulties in the isolation of sufficient...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408783/ https://www.ncbi.nlm.nih.gov/pubmed/25805862 http://dx.doi.org/10.1261/rna.047845.114 |