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SHAPE Selection (SHAPES) enrich for RNA structure signal in SHAPE sequencing-based probing data

Selective 2′ Hydroxyl Acylation analyzed by Primer Extension (SHAPE) is an accurate method for probing of RNA secondary structure. In existing SHAPE methods, the SHAPE probing signal is normalized to a no-reagent control to correct for the background caused by premature termination of the reverse tr...

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Detalles Bibliográficos
Autores principales: Poulsen, Line Dahl, Kielpinski, Lukasz Jan, Salama, Sofie R., Krogh, Anders, Vinther, Jeppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408784/
https://www.ncbi.nlm.nih.gov/pubmed/25805860
http://dx.doi.org/10.1261/rna.047068.114