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Determination of the secondary structure of group II bulge loops using the fluorescent probe 2-aminopurine
Eleven RNA hairpins containing 2-aminopurine (2-AP) in either base-paired or single nucleotide bulge loop positions were optically melted in 1 M NaCl; and, the thermodynamic parameters ΔH°, ΔS°, ΔG°(37), and T(M) for each hairpin were determined. Substitution of 2-AP for an A (adenosine) at a bulge...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cold Spring Harbor Laboratory Press
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408803/ https://www.ncbi.nlm.nih.gov/pubmed/25805856 http://dx.doi.org/10.1261/rna.048306.114 |