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Coupled local translation and degradation regulate growth cone collapse
Local translation mediates axonal responses to Semaphorin3A (Sema3A) and other guidance cues. However, only a subset of the axonal proteome is locally synthesized, while most proteins are trafficked from the soma. The reason why only specific proteins are locally synthesized is unknown. Here we show...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408908/ https://www.ncbi.nlm.nih.gov/pubmed/25901863 http://dx.doi.org/10.1038/ncomms7888 |
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author | Deglincerti, Alessia Colak, Dilek Hengst, Ulrich Liu, Yaobin Xu, Guoqiang Jaffrey, Samie R. |
author_facet | Deglincerti, Alessia Colak, Dilek Hengst, Ulrich Liu, Yaobin Xu, Guoqiang Jaffrey, Samie R. |
author_sort | Deglincerti, Alessia |
collection | PubMed |
description | Local translation mediates axonal responses to Semaphorin3A (Sema3A) and other guidance cues. However, only a subset of the axonal proteome is locally synthesized, while most proteins are trafficked from the soma. The reason why only specific proteins are locally synthesized is unknown. Here we show that local protein synthesis and degradation are linked events in growth cones. We find that growth cones exhibit high levels of ubiquitination and that local signaling pathways trigger the ubiquitination and degradation of RhoA, a mediator of Sema3A-induced growth cone collapse. Inhibition of RhoA degradation is sufficient to remove the protein-synthesis requirement for Sema3A-induced growth cone collapse. In addition to RhoA, we find that locally translated proteins are the main targets of the ubiquitin-proteasome system in growth cones. Thus, local protein degradation is a major feature of growth cones and creates a requirement for local translation to replenish proteins needed to maintain growth cone responses. |
format | Online Article Text |
id | pubmed-4408908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
record_format | MEDLINE/PubMed |
spelling | pubmed-44089082015-10-22 Coupled local translation and degradation regulate growth cone collapse Deglincerti, Alessia Colak, Dilek Hengst, Ulrich Liu, Yaobin Xu, Guoqiang Jaffrey, Samie R. Nat Commun Article Local translation mediates axonal responses to Semaphorin3A (Sema3A) and other guidance cues. However, only a subset of the axonal proteome is locally synthesized, while most proteins are trafficked from the soma. The reason why only specific proteins are locally synthesized is unknown. Here we show that local protein synthesis and degradation are linked events in growth cones. We find that growth cones exhibit high levels of ubiquitination and that local signaling pathways trigger the ubiquitination and degradation of RhoA, a mediator of Sema3A-induced growth cone collapse. Inhibition of RhoA degradation is sufficient to remove the protein-synthesis requirement for Sema3A-induced growth cone collapse. In addition to RhoA, we find that locally translated proteins are the main targets of the ubiquitin-proteasome system in growth cones. Thus, local protein degradation is a major feature of growth cones and creates a requirement for local translation to replenish proteins needed to maintain growth cone responses. 2015-04-22 /pmc/articles/PMC4408908/ /pubmed/25901863 http://dx.doi.org/10.1038/ncomms7888 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Deglincerti, Alessia Colak, Dilek Hengst, Ulrich Liu, Yaobin Xu, Guoqiang Jaffrey, Samie R. Coupled local translation and degradation regulate growth cone collapse |
title | Coupled local translation and degradation regulate growth cone collapse |
title_full | Coupled local translation and degradation regulate growth cone collapse |
title_fullStr | Coupled local translation and degradation regulate growth cone collapse |
title_full_unstemmed | Coupled local translation and degradation regulate growth cone collapse |
title_short | Coupled local translation and degradation regulate growth cone collapse |
title_sort | coupled local translation and degradation regulate growth cone collapse |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408908/ https://www.ncbi.nlm.nih.gov/pubmed/25901863 http://dx.doi.org/10.1038/ncomms7888 |
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