CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool

Engineering of the CRISPR/Cas9 system has opened a plethora of new opportunities for site-directed mutagenesis and targeted genome modification. Fundamental to this is a stretch of twenty nucleotides at the 5’ end of a guide RNA that provides specificity to the bound Cas9 endonuclease. Since a seque...

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Detalles Bibliográficos
Autores principales: Stemmer, Manuel, Thumberger, Thomas, del Sol Keyer, Maria, Wittbrodt, Joachim, Mateo, Juan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409221/
https://www.ncbi.nlm.nih.gov/pubmed/25909470
http://dx.doi.org/10.1371/journal.pone.0124633

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409221/
https://www.ncbi.nlm.nih.gov/pubmed/25909470
http://dx.doi.org/10.1371/journal.pone.0124633

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