Gel-aided sample preparation (GASP)—A simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells

We describe a “gel-assisted” proteomic sample preparation method for MS analysis. Solubilized protein extracts or intact cells are copolymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation, and matrix formation. Gel-aided sample preparation has been optimi...

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Detalles Bibliográficos
Autores principales: Fischer, Roman, Kessler, Benedikt M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2015
Materias:
Technology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409837/
https://www.ncbi.nlm.nih.gov/pubmed/25515006
http://dx.doi.org/10.1002/pmic.201400436

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409837/
https://www.ncbi.nlm.nih.gov/pubmed/25515006
http://dx.doi.org/10.1002/pmic.201400436

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