Gel-aided sample preparation (GASP)—A simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells

We describe a “gel-assisted” proteomic sample preparation method for MS analysis. Solubilized protein extracts or intact cells are copolymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation, and matrix formation. Gel-aided sample preparation has been optimi...

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Detalles Bibliográficos
Autores principales: Fischer, Roman, Kessler, Benedikt M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2015
Materias:
Technology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409837/
https://www.ncbi.nlm.nih.gov/pubmed/25515006
http://dx.doi.org/10.1002/pmic.201400436
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author Fischer, Roman
Kessler, Benedikt M
author_facet Fischer, Roman
Kessler, Benedikt M
author_sort Fischer, Roman
collection PubMed
description We describe a “gel-assisted” proteomic sample preparation method for MS analysis. Solubilized protein extracts or intact cells are copolymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation, and matrix formation. Gel-aided sample preparation has been optimized to be highly flexible, scalable, and to allow reproducible sample generation from 50 cells to milligrams of protein extracts. This methodology is fast, sensitive, easy-to-use on a wide range of sample types, and accessible to nonspecialists.
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spelling pubmed-44098372015-04-29 Gel-aided sample preparation (GASP)—A simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells Fischer, Roman Kessler, Benedikt M Proteomics Technology We describe a “gel-assisted” proteomic sample preparation method for MS analysis. Solubilized protein extracts or intact cells are copolymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation, and matrix formation. Gel-aided sample preparation has been optimized to be highly flexible, scalable, and to allow reproducible sample generation from 50 cells to milligrams of protein extracts. This methodology is fast, sensitive, easy-to-use on a wide range of sample types, and accessible to nonspecialists. BlackWell Publishing Ltd 2015-04 2015-02-05 /pmc/articles/PMC4409837/ /pubmed/25515006 http://dx.doi.org/10.1002/pmic.201400436 Text en © 2014 The Authors. PROTEOMICS published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial-NoDerivs Licence, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Technology
Fischer, Roman
Kessler, Benedikt M
Gel-aided sample preparation (GASP)—A simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells
title Gel-aided sample preparation (GASP)—A simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells
title_full Gel-aided sample preparation (GASP)—A simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells
title_fullStr Gel-aided sample preparation (GASP)—A simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells
title_full_unstemmed Gel-aided sample preparation (GASP)—A simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells
title_short Gel-aided sample preparation (GASP)—A simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells
title_sort gel-aided sample preparation (gasp)—a simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells
topic Technology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409837/
https://www.ncbi.nlm.nih.gov/pubmed/25515006
http://dx.doi.org/10.1002/pmic.201400436
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