PCR amplification of a triple-repeat genetic target directly from whole blood in 15 minutes as a proof-of-principle PCR study for direct sample analysis for a clinically relevant target

BACKGROUND: Most PCR-based diagnostics are still considered time- and labor-intensive due to disparate purification, amplification, and detection steps. Advancements in PCR enzymes and buffer chemistry have increased inhibitor tolerance, facilitating PCR directly from crude samples. Obviating the ne...

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Detalles Bibliográficos
Autores principales: Connelly, Christopher M, Porter, Laura R, TerMaat, Joel R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Technical Advance
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411754/
https://www.ncbi.nlm.nih.gov/pubmed/25495904
http://dx.doi.org/10.1186/s12881-014-0130-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411754/
https://www.ncbi.nlm.nih.gov/pubmed/25495904
http://dx.doi.org/10.1186/s12881-014-0130-5

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