A light-inducible CRISPR/Cas9 system for control of endogenous gene activation

Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing prot...

Descripción completa

Detalles Bibliográficos
Autores principales: Polstein, Lauren R., Gersbach, Charles A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4412021/
https://www.ncbi.nlm.nih.gov/pubmed/25664691
http://dx.doi.org/10.1038/nchembio.1753
_version_ 1782368590394032128
author Polstein, Lauren R.
Gersbach, Charles A.
author_facet Polstein, Lauren R.
Gersbach, Charles A.
author_sort Polstein, Lauren R.
collection PubMed
description Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.
format Online
Article
Text
id pubmed-4412021
institution National Center for Biotechnology Information
language English
publishDate 2015
record_format MEDLINE/PubMed
spelling pubmed-44120212015-09-01 A light-inducible CRISPR/Cas9 system for control of endogenous gene activation Polstein, Lauren R. Gersbach, Charles A. Nat Chem Biol Article Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes. 2015-02-09 2015-03 /pmc/articles/PMC4412021/ /pubmed/25664691 http://dx.doi.org/10.1038/nchembio.1753 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Polstein, Lauren R.
Gersbach, Charles A.
A light-inducible CRISPR/Cas9 system for control of endogenous gene activation
title A light-inducible CRISPR/Cas9 system for control of endogenous gene activation
title_full A light-inducible CRISPR/Cas9 system for control of endogenous gene activation
title_fullStr A light-inducible CRISPR/Cas9 system for control of endogenous gene activation
title_full_unstemmed A light-inducible CRISPR/Cas9 system for control of endogenous gene activation
title_short A light-inducible CRISPR/Cas9 system for control of endogenous gene activation
title_sort light-inducible crispr/cas9 system for control of endogenous gene activation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4412021/
https://www.ncbi.nlm.nih.gov/pubmed/25664691
http://dx.doi.org/10.1038/nchembio.1753
work_keys_str_mv AT polsteinlaurenr alightinduciblecrisprcas9systemforcontrolofendogenousgeneactivation
AT gersbachcharlesa alightinduciblecrisprcas9systemforcontrolofendogenousgeneactivation
AT polsteinlaurenr lightinduciblecrisprcas9systemforcontrolofendogenousgeneactivation
AT gersbachcharlesa lightinduciblecrisprcas9systemforcontrolofendogenousgeneactivation

Ejemplares similares