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A light-inducible CRISPR/Cas9 system for control of endogenous gene activation

Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing prot...

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Detalles Bibliográficos
Autores principales:	Polstein, Lauren R., Gersbach, Charles A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4412021/ https://www.ncbi.nlm.nih.gov/pubmed/25664691 http://dx.doi.org/10.1038/nchembio.1753

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