Optimal excitation and emission wavelengths to analyze amino acids and optimize neurotransmitters quantification using precolumn OPA-derivatization by HPLC

We describe an analytical methodology to obtain high sensitivity and better resolution through the study of fluorometric excitation (λ (ex)) and emission (λ (em)) spectrum wavelengths of OPA–amino acids. The spectrum emission study revealed a maximum signal peak at 450 nm for aspartate and glutamine...

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Detalles Bibliográficos
Autores principales: Perucho, J., Gonzalo-Gobernado, R., Bazan, E., Casarejos, M. J., Jiménez-Escrig, A., Asensio, M. J., Herranz, A. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2015
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4412611/
https://www.ncbi.nlm.nih.gov/pubmed/25691143
http://dx.doi.org/10.1007/s00726-015-1925-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4412611/
https://www.ncbi.nlm.nih.gov/pubmed/25691143
http://dx.doi.org/10.1007/s00726-015-1925-1

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