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New lager yeast strains generated by interspecific hybridization
The interspecific hybrid Saccharomyces pastorianus is the most commonly used yeast in brewery fermentations worldwide. Here, we generated de novo lager yeast hybrids by mating a domesticated and strongly flocculent Saccharomyces cerevisiae ale strain with the Saccharomyces eubayanus type strain. The...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4412690/ https://www.ncbi.nlm.nih.gov/pubmed/25682107 http://dx.doi.org/10.1007/s10295-015-1597-6 |
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author | Krogerus, Kristoffer Magalhães, Frederico Vidgren, Virve Gibson, Brian |
author_facet | Krogerus, Kristoffer Magalhães, Frederico Vidgren, Virve Gibson, Brian |
author_sort | Krogerus, Kristoffer |
collection | PubMed |
description | The interspecific hybrid Saccharomyces pastorianus is the most commonly used yeast in brewery fermentations worldwide. Here, we generated de novo lager yeast hybrids by mating a domesticated and strongly flocculent Saccharomyces cerevisiae ale strain with the Saccharomyces eubayanus type strain. The hybrids were characterized with respect to the parent strains in a wort fermentation performed at temperatures typical for lager brewing (12 °C). The resulting beers were analysed for sugar and aroma compounds, while the yeasts were tested for their flocculation ability and α-glucoside transport capability. These hybrids inherited beneficial properties from both parent strains (cryotolerance, maltotriose utilization and strong flocculation) and showed apparent hybrid vigour, fermenting faster and producing beer with higher alcohol content (5.6 vs 4.5 % ABV) than the parents. Results suggest that interspecific hybridization is suitable for production of novel non-GM lager yeast strains with unique properties and will help in elucidating the evolutionary history of industrial lager yeast. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10295-015-1597-6) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4412690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-44126902015-05-06 New lager yeast strains generated by interspecific hybridization Krogerus, Kristoffer Magalhães, Frederico Vidgren, Virve Gibson, Brian J Ind Microbiol Biotechnol Fermentation, Cell Culture and Bioengineering The interspecific hybrid Saccharomyces pastorianus is the most commonly used yeast in brewery fermentations worldwide. Here, we generated de novo lager yeast hybrids by mating a domesticated and strongly flocculent Saccharomyces cerevisiae ale strain with the Saccharomyces eubayanus type strain. The hybrids were characterized with respect to the parent strains in a wort fermentation performed at temperatures typical for lager brewing (12 °C). The resulting beers were analysed for sugar and aroma compounds, while the yeasts were tested for their flocculation ability and α-glucoside transport capability. These hybrids inherited beneficial properties from both parent strains (cryotolerance, maltotriose utilization and strong flocculation) and showed apparent hybrid vigour, fermenting faster and producing beer with higher alcohol content (5.6 vs 4.5 % ABV) than the parents. Results suggest that interspecific hybridization is suitable for production of novel non-GM lager yeast strains with unique properties and will help in elucidating the evolutionary history of industrial lager yeast. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10295-015-1597-6) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2015-02-15 2015 /pmc/articles/PMC4412690/ /pubmed/25682107 http://dx.doi.org/10.1007/s10295-015-1597-6 Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Fermentation, Cell Culture and Bioengineering Krogerus, Kristoffer Magalhães, Frederico Vidgren, Virve Gibson, Brian New lager yeast strains generated by interspecific hybridization |
title | New lager yeast strains generated by interspecific hybridization |
title_full | New lager yeast strains generated by interspecific hybridization |
title_fullStr | New lager yeast strains generated by interspecific hybridization |
title_full_unstemmed | New lager yeast strains generated by interspecific hybridization |
title_short | New lager yeast strains generated by interspecific hybridization |
title_sort | new lager yeast strains generated by interspecific hybridization |
topic | Fermentation, Cell Culture and Bioengineering |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4412690/ https://www.ncbi.nlm.nih.gov/pubmed/25682107 http://dx.doi.org/10.1007/s10295-015-1597-6 |
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