Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

Although the CRISPR/Cas system has enabled one-step generation of knockout mice, low success rates of cassette knock-in limit its application range. Here we show that cloning-free, direct nuclear delivery of Cas9 protein complex with chemically synthesized dual RNAs enables highly efficient target d...

Descripción completa

Detalles Bibliográficos
Autores principales: Aida, Tomomi, Chiyo, Keiho, Usami, Takako, Ishikubo, Harumi, Imahashi, Risa, Wada, Yusaku, Tanaka, Kenji F, Sakuma, Tetsushi, Yamamoto, Takashi, Tanaka, Kohichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414275/
https://www.ncbi.nlm.nih.gov/pubmed/25924609
http://dx.doi.org/10.1186/s13059-015-0653-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414275/
https://www.ncbi.nlm.nih.gov/pubmed/25924609
http://dx.doi.org/10.1186/s13059-015-0653-x

Ejemplares similares