Gold nanoparticles as a substrate in bio-analytical near-infrared surface-enhanced Raman spectroscopy
As biospectroscopy techniques continue to be developed for screening or diagnosis within a point-of-care setting, an important development for this field will be high-throughput optimization. For many of these techniques, it is therefore necessary to adapt and develop parameters to generate a robust...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Royal Society of Chemistry
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414298/ https://www.ncbi.nlm.nih.gov/pubmed/25802895 http://dx.doi.org/10.1039/c4an01899k |
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author | Butler, Holly J. Fogarty, Simon W. Kerns, Jemma G. Martin-Hirsch, Pierre L. Fullwood, Nigel J. Martin, Francis L. |
author_facet | Butler, Holly J. Fogarty, Simon W. Kerns, Jemma G. Martin-Hirsch, Pierre L. Fullwood, Nigel J. Martin, Francis L. |
author_sort | Butler, Holly J. |
collection | PubMed |
description | As biospectroscopy techniques continue to be developed for screening or diagnosis within a point-of-care setting, an important development for this field will be high-throughput optimization. For many of these techniques, it is therefore necessary to adapt and develop parameters to generate a robust yet simple approach delivering high-quality spectra from biological samples. Specifically, this is important for surface-enhanced Raman spectroscopy (SERS) wherein there are multiple variables that can be optimised to achieve an enhancement of the Raman signal from a sample. One hypothesis is that “large” diameter (>100 nm) gold nanoparticles provide a greater enhancement at near-infrared (NIR) and infrared (IR) wavelengths than those <100 nm in diameter. Herein, we examine this notion using examples in which SERS spectra were acquired from MCF-7 breast cancer cells incubated with 150 nm gold nanoparticles. It was found that 150 nm gold nanoparticles are an excellent material for NIR/IR SERS. Larger gold nanoparticles may better satisfy the theoretical restraints for SERS enhancement at NIR/IR wavelengths compared to smaller nanoparticles. Also, larger nanoparticles or their aggregates are more readily observed via optical microscopy (and especially electron microscopy) compared to smaller ones. This allows rapid and straightforward identification of target areas containing a high concentration of nanoparticles and facilitating SERS spectral acquisition. To some extent, these observations appear to extend to biofluids such as blood plasma or (especially) serum; SERS spectra of such biological samples often exhibit a low signal-to-noise ratio in the absence of nanoparticles. With protein-rich biofluids such as serum, a dramatic SERS effect can be observed; although this might facilitate improved spectral biomarker identification in the future, it may not always improve classification between control vs. cancer. Thus, use of “large” gold nanoparticles are a good starting point in order to derive informative NIR/IR SERS analysis of biological samples. |
format | Online Article Text |
id | pubmed-4414298 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-44142982015-05-22 Gold nanoparticles as a substrate in bio-analytical near-infrared surface-enhanced Raman spectroscopy Butler, Holly J. Fogarty, Simon W. Kerns, Jemma G. Martin-Hirsch, Pierre L. Fullwood, Nigel J. Martin, Francis L. Analyst Chemistry As biospectroscopy techniques continue to be developed for screening or diagnosis within a point-of-care setting, an important development for this field will be high-throughput optimization. For many of these techniques, it is therefore necessary to adapt and develop parameters to generate a robust yet simple approach delivering high-quality spectra from biological samples. Specifically, this is important for surface-enhanced Raman spectroscopy (SERS) wherein there are multiple variables that can be optimised to achieve an enhancement of the Raman signal from a sample. One hypothesis is that “large” diameter (>100 nm) gold nanoparticles provide a greater enhancement at near-infrared (NIR) and infrared (IR) wavelengths than those <100 nm in diameter. Herein, we examine this notion using examples in which SERS spectra were acquired from MCF-7 breast cancer cells incubated with 150 nm gold nanoparticles. It was found that 150 nm gold nanoparticles are an excellent material for NIR/IR SERS. Larger gold nanoparticles may better satisfy the theoretical restraints for SERS enhancement at NIR/IR wavelengths compared to smaller nanoparticles. Also, larger nanoparticles or their aggregates are more readily observed via optical microscopy (and especially electron microscopy) compared to smaller ones. This allows rapid and straightforward identification of target areas containing a high concentration of nanoparticles and facilitating SERS spectral acquisition. To some extent, these observations appear to extend to biofluids such as blood plasma or (especially) serum; SERS spectra of such biological samples often exhibit a low signal-to-noise ratio in the absence of nanoparticles. With protein-rich biofluids such as serum, a dramatic SERS effect can be observed; although this might facilitate improved spectral biomarker identification in the future, it may not always improve classification between control vs. cancer. Thus, use of “large” gold nanoparticles are a good starting point in order to derive informative NIR/IR SERS analysis of biological samples. Royal Society of Chemistry 2015-05-07 2015-03-24 /pmc/articles/PMC4414298/ /pubmed/25802895 http://dx.doi.org/10.1039/c4an01899k Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Chemistry Butler, Holly J. Fogarty, Simon W. Kerns, Jemma G. Martin-Hirsch, Pierre L. Fullwood, Nigel J. Martin, Francis L. Gold nanoparticles as a substrate in bio-analytical near-infrared surface-enhanced Raman spectroscopy |
title | Gold nanoparticles as a substrate in bio-analytical near-infrared surface-enhanced Raman spectroscopy
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title_full | Gold nanoparticles as a substrate in bio-analytical near-infrared surface-enhanced Raman spectroscopy
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title_fullStr | Gold nanoparticles as a substrate in bio-analytical near-infrared surface-enhanced Raman spectroscopy
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title_full_unstemmed | Gold nanoparticles as a substrate in bio-analytical near-infrared surface-enhanced Raman spectroscopy
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title_short | Gold nanoparticles as a substrate in bio-analytical near-infrared surface-enhanced Raman spectroscopy
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title_sort | gold nanoparticles as a substrate in bio-analytical near-infrared surface-enhanced raman spectroscopy |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414298/ https://www.ncbi.nlm.nih.gov/pubmed/25802895 http://dx.doi.org/10.1039/c4an01899k |
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