Functional annotation of native enhancers with a Cas9 -histone demethylase fusion

Understanding of mammalian enhancer function is limited by the lack of a technology to rapidly and thoroughly test their cell type-specific function. Here, we use a nuclease-deficient (d)Cas9 histone demethylase fusion to functionally characterize previously described and novel enhancer elements for...

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Detalles Bibliográficos
Autores principales: Kearns, Nicola A, Pham, Hannah, Tabak, Barbara, Genga, Ryan M, Silverstein, Noah J, Garber, Manuel, Maehr, René
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414811/
https://www.ncbi.nlm.nih.gov/pubmed/25775043
http://dx.doi.org/10.1038/nmeth.3325
Descripción
Sumario:Understanding of mammalian enhancer function is limited by the lack of a technology to rapidly and thoroughly test their cell type-specific function. Here, we use a nuclease-deficient (d)Cas9 histone demethylase fusion to functionally characterize previously described and novel enhancer elements for their roles in the embryonic stem cell state. Further, we distinguish the mechanism of action of dCas9-LSD1 at enhancers from previous dCas9-effectors.

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