Functional annotation of native enhancers with a Cas9 -histone demethylase fusion

Understanding of mammalian enhancer function is limited by the lack of a technology to rapidly and thoroughly test their cell type-specific function. Here, we use a nuclease-deficient (d)Cas9 histone demethylase fusion to functionally characterize previously described and novel enhancer elements for...

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Autores principales: Kearns, Nicola A, Pham, Hannah, Tabak, Barbara, Genga, Ryan M, Silverstein, Noah J, Garber, Manuel, Maehr, René
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414811/
https://www.ncbi.nlm.nih.gov/pubmed/25775043
http://dx.doi.org/10.1038/nmeth.3325
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author Kearns, Nicola A
Pham, Hannah
Tabak, Barbara
Genga, Ryan M
Silverstein, Noah J
Garber, Manuel
Maehr, René
author_facet Kearns, Nicola A
Pham, Hannah
Tabak, Barbara
Genga, Ryan M
Silverstein, Noah J
Garber, Manuel
Maehr, René
author_sort Kearns, Nicola A
collection PubMed
description Understanding of mammalian enhancer function is limited by the lack of a technology to rapidly and thoroughly test their cell type-specific function. Here, we use a nuclease-deficient (d)Cas9 histone demethylase fusion to functionally characterize previously described and novel enhancer elements for their roles in the embryonic stem cell state. Further, we distinguish the mechanism of action of dCas9-LSD1 at enhancers from previous dCas9-effectors.
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spelling pubmed-44148112015-11-01 Functional annotation of native enhancers with a Cas9 -histone demethylase fusion Kearns, Nicola A Pham, Hannah Tabak, Barbara Genga, Ryan M Silverstein, Noah J Garber, Manuel Maehr, René Nat Methods Article Understanding of mammalian enhancer function is limited by the lack of a technology to rapidly and thoroughly test their cell type-specific function. Here, we use a nuclease-deficient (d)Cas9 histone demethylase fusion to functionally characterize previously described and novel enhancer elements for their roles in the embryonic stem cell state. Further, we distinguish the mechanism of action of dCas9-LSD1 at enhancers from previous dCas9-effectors. 2015-03-16 2015-05 /pmc/articles/PMC4414811/ /pubmed/25775043 http://dx.doi.org/10.1038/nmeth.3325 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Kearns, Nicola A
Pham, Hannah
Tabak, Barbara
Genga, Ryan M
Silverstein, Noah J
Garber, Manuel
Maehr, René
Functional annotation of native enhancers with a Cas9 -histone demethylase fusion
title Functional annotation of native enhancers with a Cas9 -histone demethylase fusion
title_full Functional annotation of native enhancers with a Cas9 -histone demethylase fusion
title_fullStr Functional annotation of native enhancers with a Cas9 -histone demethylase fusion
title_full_unstemmed Functional annotation of native enhancers with a Cas9 -histone demethylase fusion
title_short Functional annotation of native enhancers with a Cas9 -histone demethylase fusion
title_sort functional annotation of native enhancers with a cas9 -histone demethylase fusion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414811/
https://www.ncbi.nlm.nih.gov/pubmed/25775043
http://dx.doi.org/10.1038/nmeth.3325
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