Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrP(Sc) over time

Ovine scrapie can be transmitted via environmental reservoirs. A pool of ovine scrapie isolates were incubated on soil for one day or thirteen months and eluted prion was used to challenge tg338 mice transgenic for ovine PrP. After one-day incubation on soil, two PrP(Sc) phenotypes were present: G(3...

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Autores principales: Maddison, Ben C, Spiropoulos, John, Vickery, Christopher M, Lockey, Richard, Owen, Jonathan P, Bishop, Keith, Baker, Claire A, Gough, Kevin C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415298/
https://www.ncbi.nlm.nih.gov/pubmed/25928902
http://dx.doi.org/10.1186/s13567-015-0179-y
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author Maddison, Ben C
Spiropoulos, John
Vickery, Christopher M
Lockey, Richard
Owen, Jonathan P
Bishop, Keith
Baker, Claire A
Gough, Kevin C
author_facet Maddison, Ben C
Spiropoulos, John
Vickery, Christopher M
Lockey, Richard
Owen, Jonathan P
Bishop, Keith
Baker, Claire A
Gough, Kevin C
author_sort Maddison, Ben C
collection PubMed
description Ovine scrapie can be transmitted via environmental reservoirs. A pool of ovine scrapie isolates were incubated on soil for one day or thirteen months and eluted prion was used to challenge tg338 mice transgenic for ovine PrP. After one-day incubation on soil, two PrP(Sc) phenotypes were present: G(338) or Apl(338)ii. Thirteen months later some divergent PrP(Sc) phenotypes were seen: a mixture of Apl(338)ii with either G(338) or P(338,) and a completely novel PrP(Sc) deposition, designated Cag(338). The data show that prolonged ageing of scrapie prions within an environmental matrix may result in changes in the dominant PrP(Sc) biological/biochemical properties.
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spelling pubmed-44152982015-05-01 Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrP(Sc) over time Maddison, Ben C Spiropoulos, John Vickery, Christopher M Lockey, Richard Owen, Jonathan P Bishop, Keith Baker, Claire A Gough, Kevin C Vet Res Short Report Ovine scrapie can be transmitted via environmental reservoirs. A pool of ovine scrapie isolates were incubated on soil for one day or thirteen months and eluted prion was used to challenge tg338 mice transgenic for ovine PrP. After one-day incubation on soil, two PrP(Sc) phenotypes were present: G(338) or Apl(338)ii. Thirteen months later some divergent PrP(Sc) phenotypes were seen: a mixture of Apl(338)ii with either G(338) or P(338,) and a completely novel PrP(Sc) deposition, designated Cag(338). The data show that prolonged ageing of scrapie prions within an environmental matrix may result in changes in the dominant PrP(Sc) biological/biochemical properties. BioMed Central 2015-05-01 2015 /pmc/articles/PMC4415298/ /pubmed/25928902 http://dx.doi.org/10.1186/s13567-015-0179-y Text en © Maddison et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Maddison, Ben C
Spiropoulos, John
Vickery, Christopher M
Lockey, Richard
Owen, Jonathan P
Bishop, Keith
Baker, Claire A
Gough, Kevin C
Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrP(Sc) over time
title Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrP(Sc) over time
title_full Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrP(Sc) over time
title_fullStr Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrP(Sc) over time
title_full_unstemmed Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrP(Sc) over time
title_short Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrP(Sc) over time
title_sort incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of prp(sc) over time
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415298/
https://www.ncbi.nlm.nih.gov/pubmed/25928902
http://dx.doi.org/10.1186/s13567-015-0179-y
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