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Sonication, Vacuum Infiltration and Thiol Compounds Enhance the Agrobacterium-Mediated Transformation Frequency of Withania somnifera (L.) Dunal

In the present study, we have established a stable transformation protocol via Agrobacterium tumafacines for the pharmaceutically important Withania somnifera. Six day-old nodal explants were used for 3 day co-cultivation with Agrobacterium tumefaciens strain LBA4404 harbouring the vector pCAMIBA230...

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Detalles Bibliográficos
Autores principales: Sivanandhan, Ganeshan, Kapil Dev, Gnajothi, Theboral, Jeevaraj, Selvaraj, Natesan, Ganapathi, Andy, Manickavasagam, Markandan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416026/
https://www.ncbi.nlm.nih.gov/pubmed/25927703
http://dx.doi.org/10.1371/journal.pone.0124693