Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast

Transformation-associated recombination (TAR) protocol allowing the selective isolation of full-length genes complete with their distal enhancer regions and entire genomic loci with sizes up to 250 kb from complex genomes in yeast S. cerevisiae has been developed more than a decade ago. However, its...

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Detalles Bibliográficos
Autores principales: Lee, Nicholas C.O., Larionov, Vladimir, Kouprina, Natalay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417148/
https://www.ncbi.nlm.nih.gov/pubmed/25690893
http://dx.doi.org/10.1093/nar/gkv112

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417148/
https://www.ncbi.nlm.nih.gov/pubmed/25690893
http://dx.doi.org/10.1093/nar/gkv112

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