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RNA editing by T7 RNA polymerase bypasses InDel mutations causing unexpected phenotypic changes
DNA-dependent T7 RNA polymerase (T7 RNAP) is the most powerful tool for both gene expression and in vitro transcription. By using a Next Generation Sequencing (NGS) approach we have analyzed the polymorphism of a T7 RNAP-generated mRNA pool of the mboIIM2 gene. We find that the enzyme displays a rel...
Autores principales: | Wons, Ewa, Furmanek-Blaszk, Beata, Sektas, Marian |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417176/ https://www.ncbi.nlm.nih.gov/pubmed/25824942 http://dx.doi.org/10.1093/nar/gkv269 |
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