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Role of N-linked glycosylation in the secretion and enzymatic properties of Rhizopus chinensis lipase expressed in Pichia pastoris

BACKGROUND: The methylotrophic yeast, Pichia pastoris, is widely used as a useful experimental tool in protein engineering and production. It is common for proteins expressed in P. pastoris to exhibit N-glycosylation. In recent years, glycosylation studies in P. pastoris have attracted increasing at...

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Detalles Bibliográficos
Autores principales: Yang, Min, Yu, Xiao-Wei, Zheng, Haiyan, Sha, Chong, Zhao, Caifeng, Qian, Meiqian, Xu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417512/
https://www.ncbi.nlm.nih.gov/pubmed/25880561
http://dx.doi.org/10.1186/s12934-015-0225-5